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. 2022 Jan 24;18(4):1491–1507. doi: 10.7150/ijbs.69351

Figure 6.

Figure 6

PCDH7 is a target of miR-412-3p and suppresses ccRCC proliferation, migration and invasion. (A) Volcano plot showing downstream genes with significantly altered expression in 786-O cell overexpressing miR-412-3p and miR-NC mimics. (B) Schematic illustration exhibiting the overlap between the target mRNAs and miR-412-3p as detected by TargetScan, miRDB, and RNA-seq. (C) 786-O and ACHN were transfected with miR-412-3p or miR-NC mimics for 48 h, and the expression of downstream genes was analyzed by qPCR. (D) The sequence of WT and MUT putative miR-412-3p-binding sites in the 3'-UTR of PCDH7. (E) Relative luciferase activities were examined in 293T after transfection with PCDH7-WT or PCDH7-MUT and miR-412-3p mimic or miR-NC. (F) Western blot results of PCDH7 levels in ccRCC cell lines after transfection with miR-412-3p mimic or miR-NC. (G) PCDH7 expression in 786-O, ACHN, and 293T was analyzed by RT-qPCR. (H) The relative abundance of PCDH7 in 786-O and ACHN cells transfected with si-NC and si-PCDH7 was determined by qRT-PCR. (I) Western blot of PCDH7 levels in ccRCC cell lines after transfection si-NC and si-PCDH7. (J) Colony formation ability of 786-O and ACHN cells transfected with si-NC and si-PCDH7. (K) The proliferation ability of 786-O and ACHN transfected with si-NC and si-PCDH7 was tested by the CCK-8 assay. (L) Transwell migration and invasion assays indicated that si-PCDH7 promoted migration and invasion capacity of transfected 786-O and ACHN. Scale bar, 100 µm. si-NC, siRNA for NC. si-PCDH7, siRNA against PCDH7. miR-NC mimic was used as the miRNA mimic NC. Data are exhibited as mean ± SD. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, no significant (NS).