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. 2022 Jan 24;18(4):1539–1554. doi: 10.7150/ijbs.67842

Figure 2.

Figure 2

PANK1 inhibits proliferation, growth, and invasion of HCC cells. (A) PANK1 was overexpressed in MHCC97H, Huh7, QGY-7701and PVTT cells. MHCC97H, Huh7, QGY-7701 and PVTT cells were infected with a lentivirus with overexpressed Flag-PANK1. After puromycin screening, the expression of Flag-PANK1 in the surviving cells was measured by western blot using anti-Flag or anti-PANK1 antibodies. (B) A CCK8 assay was performed to measure the effect of PANK1 overexpression on the growth of PVTT and QGY-7701 cells. (C-D) EdU assay was performed to examine the effect of PANK1 expression on the proliferation of PVTT and QGY-7701 cells. The positive cells were quantified and analyzed. (E-F) A soft agar colony formation assay was performed to examine the effect of PANK1 expression on PVTT, MHCC97H, QGY-7701 and Huh7 colonies. The colonies were quantified and analyzed. (G-H) Invasion assay using the Transwell was conducted to examine the effect of PANK1 expression on the invasion of PVTT, QGY-7701 and MHCC97H cells. The invasive cells were quantified and analyzed. The scale bars were indicated. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.