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. 2022 Jan 24;18(4):1539–1554. doi: 10.7150/ijbs.67842

Figure 3.

Figure 3

Knocking down PANK1 promotes proliferation, growth, colony formation, and invasion of HCC cells. (A) PANK1 expression was knocked down in Huh7 and QGY-7701 cells. The cells were infected with the shPANK1 lentivirus. After puromycin screening, expression of PANK1 in the surviving cells was measured by western blot. (B) A CCK8 assay was performed to measure the effect of interfering with PANK1 on growth of the HCC cells Huh7 and QGY-7701. (C-D) EdU assay was performed to quantify and analyze the effect of knocking down PANK1 expression on the proliferation of Huh7 and QGY-7701 cells. (E-F) A soft agar colony formation assay was performed to quantify and analyze the effect of knocking down PANK1 expression on the colony formation of Huh7 and QGY-7701 cells. (G-H) Invasion assay using the Transwell was conducted to quantify and analyze the effect of knocking down PANK1 expression on the migration ability of Huh7 and QGY-7701 cells. The scale bars were indicated. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.