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. 2022 Jan 16;18(4):1381–1397. doi: 10.7150/ijbs.65476

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BBR protects TNF-α induced Caco2 cell damages via Wnt/β-catenin pathway. (A) The transfection efficiency of siRNA on the expression of Wnt and β-catenin was measured by western blotting. (B) Grey values of Wnt and β-catenin normalized to GAPDH. (C) The mRNA levels of Wnt and β-catenin in transfected cells measured by qPCR. (D) The protein levels of Occludin and ZO-1 in Caco2 cells with transfection were measured by western blotting. (E) Grey values of Occludin and ZO-1 normalized to GAPDH. Data are presented as mean ± SD. *P< 0.05, significantly different as indicated.