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. Author manuscript; available in PMC: 2022 Mar 6.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Apr 28;32(9):2358–2365. doi: 10.1021/jasms.1c00079

Figure 5.

Figure 5.

Mitochondrial TurboID proteomics with thiol-cleavable biotin. (A) Schematic of STOML2-TurboID inside the mitochondrion using thiol-cleavable SS-biotin as the substrate. (B) Volcano plot of STOML2-TurboID proteomics vs negative control (N = 3). Significantly enriched proteins are highlighted in red. Endogenously biotinylated carboxylases were highlighted in green. (C) Go-term analysis of significantly enriched proteins (top 4 ranked Go-terms for cellular location). Mitochondrial structure: outer mitochondrial membrane (OMM), intermembrane space (IMS), inner mitochondrial membrane (IMM), and mitochondrial matrix.