Skip to main content
. Author manuscript; available in PMC: 2022 Mar 6.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Apr 28;32(9):2358–2365. doi: 10.1021/jasms.1c00079

Table 1.

Reaction Efficiency of SS Cleavage and Carbomethylation from SS-Biotin-Labeled (K) Human Cell Lysate Proteins

modification Δ mass biotinylated peptides (%) avg percolator q-value avg percolator PEP
cleaved, carbomethylateda +145.0198 99.5 8.90 × 10−04 1.47 × 10−02
cleaved, not carbomethylatedb +87.9983 0.31 5.50 × 10−03 1.22 × 10−01
SS-biotinylated, not cleavedb +389.0902 0.20 2.50 × 10−04 8.46 × 10−04
a

Complete reaction of thiol-cleavable biotinylation.

b

Incomplete reaction of thiol-cleavable biotinylation.