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. 2022 Mar 5;23:182. doi: 10.1186/s12864-022-08426-7

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© The Author(s) 2022, corrected publication 2022

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Fig. 2 — Potential HGT Region and nadK Phylogenetic Tree. A) Highly similar genomic regions containing the equol production gene cluster in Slackia_A equolifaciens, Enteroscipio sp000270285, and L. garvieae 20–92. Homologous regions between the nucleotide sequences are shown as shaded areas, with darker coloring meaning the genes had a high identity and the identity values being shown as the numbers in each shaded area. The region containing the equol gene cluster is highlighted with a red bar on the Slackia_A equolifaciens sequence. B) A phylogenetic tree based on the nadK homolog sequences identified in Coriobacteriales genomes. Genes from genera that are from species with identified equol gene clusters are colored by genus, and the nadK homologs from the Slackia_A equolifaciens and Enteroscipio sp000270285 equol gene clusters are highlighted with yellow backgrounds. Genes from the Coriobacteriaceae, Atopobiaceae, and UMGS124 families were collapsed into a single leaf shown as a gray triangle. Branches leading to genes from the Eggerthellaceae family are colored blue. All bootstrap support values greater than 75% are shown