Fig. 5.

Characterization of transgene induction in stable transgenic lines. a Injected F0 fish have mosaic expression of the transgene. F0 founders transmit the transgene through the germ line to a small fraction of F1 progeny. b Crosses of transgene positive F1 fish results in 3/4 of the progeny carrying at least 1 copy of the transgene. c Luciferase activity was detectable in induced stable line embryos, but at lower levels when compared to injected embryos, although levels were still within the linear range of the assay. There was no visible GFP expression in stable line induced embryos. Transgene expression is diminished at concentrations of HgCl₂ that are correlated with increased mortality. Results are the average values from 3 groups of 8 for each treatment. Error bars represent standard error of the mean