FIG. 2.

(A and B) OL cells mock infected or persistently infected with HuP2br were immunohistochemically stained with polyclonal rabbit anti-BDV p40 and then with fluorescein isothiocyanate-conjugated goat anti-rabbit IgG (A) and were subjected to in situ hybridization using antisense digoxigenin-conjugated riboprobe directed to BDV p40 region and then stained with fluorescent-labeled anti-digoxigenin Ig (B). (C) Newborn gerbils were mock infected or infected with HuP2Br. The sections prepared from the cerebral cortex (frontal lobe) of the gerbils on day 30 postinfection were subjected to in situ hybridization using antisense riboprobe directed to the BDV p40 region. Figure courtesy of Kazuyoshi Ikuta, Osaka University, Osaka, Japan.