FIGURE 4.

Anti‐programmed cell death‐ligand 1 (PD‐L1) treatment leads to increase in M1 macrophage infiltration in Lewis lung cancer cells (LLC) tumors following NLRP4 knockdown (KD). A, Cell composition analysis in LLC‐derived grafted tumors by CIBERSORT method. B, Phenotypic analysis of Raw 264.7 cells cocultured with NC‐LLC and NLRP4 KD‐LLC cells. Data are the representative of at least two independent experiments. C, D, Flow cytometric analysis of CD11b⁺F4/80⁺CD86⁺CD206⁻ (M1) and CD11b⁺F4/80⁺CD206⁺CD86⁻ (M2) macrophages in LLC inoculated tumors. E‐G, Comparisons of M2 (E) and M1 (F) proportions in CD11b⁺F4/80⁺ macrophages and PD‐L1 expression on M2 macrophages (G) in LLC inoculated tumors. H‐J, Immunohistochemical results of Arginase 1 (Arg1) (H), CD206 (I), and inducible nitric oxide synthase (iNOS) (J) in LLC inoculated tumors. Data are shown as mean ± SEM; n = 4‐8 mice per group. P values were calculated using ANOVA and Student’s t test. *P < .05; **P < .01; ****P < .0001. DC, dendritic cell; NC, negative control; NK, natural killer