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. 2021 Dec 30;113(3):904–915. doi: 10.1111/cas.15246

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© 2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Organoid cultures to recapitulate in vivo‐like differentiation. Organoids were generated with the indicated HCK1T cell lines in the absence of doxycycline. Formalin‐fixed paraffin‐embedded sections of organoids were subjected to H&E staining and Alcian blue/periodic acid‐Schiff (AB/PAS) staining (A) or immunohistochemistry for p40 (ΔNp63α), cytokeratin (CK)14, CK18, MUC5AC, and Ki‐67 (B). Fractions of AB/PAS‐positive organoids were calculated based on manual counting and indicated in (A). Arrows indicate mucin‐like substance. Scale bar, 100 µm