REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Human NTD mAb 4A8	(Chi et al., 2020)	N/A
Human RBD mAb COVA2-15	(Brouwer et al., 2020)	N/A
Human RBD mAb BD368-2	(Cao et al., 2020)	N/A
Human RBD mAb S2M11	(Tortorici et al., 2020)	N/A
Human RBD mAb REGN10933	(Hansen et al., 2020)	N/A
Mouse RBD mAb 36H6	(Zhang et al., 2021)	N/A
Mouse RBD mAb 3C8	(Zhang et al., 2021)	N/A
Mouse RBD mAb 2B4	(Zhang et al., 2021)	N/A
Mouse RBD mAb 85F7	(Wu et al., 2022)	N/A
Biological samples
Human COVID-19 convalescent plasmas	(Chang et al., 2021; Zhang et al., 2021)	Table S2
Recombinant spike-protein immunized animal sera	(Wu et al., 2021)	N/A
Recombinant RBD-protein immunized animal sera	(Wu et al., 2021)	N/A
Chemicals, peptides, and recombinant proteins
rACE2 protein of human	This study	N/A
rACE2 protein of monkey	This study	N/A
rACE2 protein of tupaia	This study	N/A
rACE2 protein of cat	This study	N/A
rACE2 protein of puma	This study	N/A
rACE2 protein of dog	This study	N/A
rACE2 protein of ferret	This study	N/A
rACE2 protein of rabbit	This study	N/A
rACE2 protein of hamster	This study	N/A
rACE2 protein of mouse	This study	N/A
rACE2 protein of horse	This study	N/A
rACE2 protein of camel	This study	N/A
rACE2 protein of pig	This study	N/A
rACE2 protein of pangolin	This study	N/A
rACE2 protein of horseshoe bat	This study	N/A
rACE2 protein of whale	This study	N/A
rACE2 protein of sea lion	This study	N/A
rACE2 protein of brown trout	This study	N/A
SARS-CoV2-STG	(Zhang et al., 2021)	N/A
StriFK protein	(Wu et al., 2021)	N/A
S-HexaPro protein	(Hsieh et al., 2020)	N/A
Critical commercial assays
Nano Glo HiBiT Extracellular Detection System	Promega	Cat# N2421
Nano Glo HiBiT Lytic Detection System	Promega	Cat# N3040
HIV-4 Ag/Ab ELISA kit	Beijing Wantai BioPharm	Cat# S20080008
Lipofectamine® 3000 Transfection Reagent	Thermo Scientific	Cat# L3000-015
ExpiFectamine™ CHO Transfection Kit	Thermo Scientific	Cat# A29129
Ni Sepharose 6 Fast Flow	Cytiva	Cat# 17-5318-03
MabSelect SuRe resin	Cytiva	Cat# 17-5474-02
Experimental models: Cell lines
ExpiCHO-S cells	Thermo Scientific	Cat# A29127; RRID: CVCL_5J31
H1299 cells	ATCC	Cat# CRL-5803; RRID: CVCL_0060
HEK293T/17 cells	ATCC	Cat# CRL-11268; RRID: CVCL_1926
huACE2-H1299 (human ACE2)	(Zhang et al., 2021)	N/A
moACE2-H1299 (monkey ACE2)	This study	N/A
tuACE2-H1299 (tupaia ACE2)	This study	N/A
catACE2-H1299 (cat ACE2)	This study	N/A
puACE2-H1299 (puma ACE2)	This study	N/A
dogACE2-H1299 (dog ACE2)	This study	N/A
feACE2-H1299 (ferret ACE2)	This study	N/A
rabACE2-H1299 (rabbit ACE2)	This study	N/A
haACE2-H1299 (hamster ACE2)	This study	N/A
muACE2-H1299 (mouse ACE2)	This study	N/A
hoACE2-H1299 (horse ACE2)	This study	N/A
camACE2-H1299 (camel ACE2)	This study	N/A
pigACE2-H1299 (pig ACE2)	This study	N/A
pagACE2-H1299 (pangolin ACE2)	This study	N/A
hbACE2-H1299 (horseshoe bat ACE2)	This study	N/A
whACE2-H1299 (whale ACE2)	This study	N/A
slionACE2-H1299 (sea lion ACE2)	This study	N/A
btACE2-H1299 (brown trout ACE2)	This study	N/A
Recombinant DNA
EIRBsMie-dSwtG(S614G)	This study	N/A
EIRBsMie-dSwtD(S614D)	This study	N/A
EIRBsMie-SARS1rbd	This study	N/A
Plasmid panel: EIRBsMie-dSwtG based single-site RBD mutants (n=129)	This study	N/A
Plasmid panel: EIRBsMie-dSwtG based double-site RBD mutants (n=24)	This study	N/A
ACE2 orthologs cDNA	GenBank	Table S1
Plasmid panel: pLVEF1αIHRB-ACE2 orthologs panel (n=18)	This study	N/A
EIRBsMie-dSuk1201G(Alpha/B.1.1.7)	This study	N/A
EIRBsMie-dSBR1216(Gamma/P.1)	This study	N/A
EIRBsMie-dSSA118(Beta/B.1.351)	This study	N/A
EIRBsMie-dSCAL20C (Epsilon/B.1.429)	This study	N/A
EIRBsMie-dSuk1188 (Eta/B.1.525)	This study	N/A
EIRBsMie-dSus1123A (Iota_477N/B.1.526a)	This study	N/A
EIRBsMie-dSus1123B (Iota_484K/B.1.526b)	This study	N/A
EIRBsMie-dSIND288 (Kappa/B.1.617.1)	This study	N/A
EIRBsMie-dSIND682 (Delta/B.1.617.2)	This study	N/A
EIRBsMie-dSOLV639 (B.1.620)	This study	N/A
EIRBsMie-dSSA668 (Omicron/B.1.1.529)	This study	N/A
psPAX2	Addgene	RRID: Addgene_12260
pLVEF1αmNG	(Zhang et al., 2021)	N/A
Software and algorithms
Columbus Analysis system (version 2.5.0)	PerkinElmer	https://www.perkinelmer.com/
GraphPad Prism (version 8.0.1/9.0.0)	Graphpad	https://www.graphpad.com/
FlowJo (version 10.6.0)	FlowJo	https://www.flowjo.com/
Origin 2021	OriginLab	https://www.originlab.com/2021
Leica LAS X (Version 3.5.5)	Leica	https://www.leicamicrosystems.com
SAS (version 9.4)	SAS	https://www.sas.com/
Python (version 3.9.6)	Python	https://www.python.org/downloads/release/python-396/