Figure 4.
Naringin inhibits TNF-α-induced human NP cell apoptosis. (a) MitoTracker Red CMXRos fluorescence under fluorescence microscope (scale bar = 100 μm). (b) Annexin-V/PI staining to detect the rate of apoptosis in human NP cells. (c) The protein levels of C-caspase-3, Bax, and Beclin-2 in cells were measured by Western blotting. The data represent the means ± SD. Significant differences between groups are indicated as ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001, n = 3.