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Renal NADPH oxidase subunits were activated in diabetic mice subjected to LPS. Renal NADPH subunits, NOX2 and NOX4, were detected by western blotting (a). Gray analysis was performed according to the bands of NOX2 (b) and NOX4 (c). Renal malondialdehyde (MDA) (d) expression was measured using enzyme-linked immunosorbent assay (ELISA). Each bar represents the mean ± SEM (n = 3 − 8). ∗p < 0.05 and ∗∗p < 0.01, one-way ANOVA with Tukey's test.
