Figure 9.

Inhibition of the NADPH oxidase pathway attenuated renal ferroptosis accumulation. Ferroptosis-related proteins, including ASCL4, FTH1, and GPX4, were determined by western blotting (a). GPX4 levels were also detected by immunohistochemical staining (b). The renal scavenging effect on ROS is reflected by the glutathione reductase (GR) level (c). Morphological features of mitochondria were measured using electron microscopy (d). Each bar represents the mean ± SEM (n = 3 − 8). ^∗p < 0.05 and ^∗∗p < 0.01, one-way ANOVA with Tukey's test.