Figure 7.

CREB in the PTH/PTH1R signalling pathway bound the promoter sequence of SHH and promoted SHH expression. (a) The immunoprecipitates bound by the CREB antibody were used for PCR. (b) The SHH promoter sequence was as shown. The part marked in red is the predicted binding site. WT and relative mutated sequences were constructed with pGL4.23. (c) The relative Luc activity derived by NC, WT, and MUT-SHH promoter sequences in human NP cells. Data are presented as mean ± SD (n = 6). ^∗∗∗p < 0.001.