TABLE 1.
Essential slit diaphragm proteins.
| Human protein | Fly ortholog | DIOPT score | Protein function | Function in podocytes/nephrocytes |
|---|---|---|---|---|
| Nephrin | Sns | 14 | Cell junction | SD Component |
| NEPH1 | Duf | 11 | Cell junction | SD Component |
| ZO-1 | Pyd | 11 | Cell junction | SD Component |
| CD2AP | Cindr | 8 | Cell junction | SD Component |
| Podocin | Mec2 | 3 | Cell junction | SD Component |
| PRKCI (aPKC) | aPKC | 12 | Cell polarity | SD Formation |
| PARD6 (G/B/A) | Par-6 | 12 | Cell polarity | SD Formation |
| PARD3 | Baz | 13 | Cell polarity | SD Formation |
| DLG1 | Dlg | 13 | Cell polarity | SD Formation |
| LLGL1 | Lgl | 15 | Cell polarity | SD Formation |
| SCRIB | Scrib | 7 | Cell polarity | SD Formation |
| MARK1 | Par-1 | 10 | Cell polarity | SD Formation |
| STK11 (LKB1) | Lkb1 | 12 | Cell polarity | SD Formation |
| CRB1 | Crb | 9 | Cell polarity | SD Formation |
| PALS1 (MPP5) | Sdt | 12 | Cell polarity | SD Formation |
| PATJ | Patj | 9 | Cell polarity | SD Formation |
| EZR/RDX/MSN | Moe | 13/13/14 | Actin regulation | Cytoskeleton |
| ARHGDIA | RhoGDI | 15 | Actin regulation | Cytoskeleton |
| ARHGAP24 | RhoGAP92B | 1 | Actin regulation | Cytoskeleton |
| KANK1, 2, 4 | Kank | 10 | Actin regulation | Cytoskeleton |
| MYH9 | Zip | 11 | Actin regulation | Cytoskeleton |
| ACTN4 | Actn | 11 | Actin regulation | Cytoskeleton |
| MYO1E | Myo61F | 3 | Actin regulation | Cytoskeleton |
| INF2 | Form3 | 7 | Actin regulation | Cytoskeleton |
| CLTA | Chc | 13 | Endocytosis | SD Maintenance |
| CLTC | Clc | 14 | Endocytosis | SD Maintenance |
| DYNAMIN | Shi | 13 | Endocytosis | SD Maintenance |
| AP2B1 | AP-1-2β | 12 | Endocytosis | SD Maintenance |
| AP2A2 | AP-2α | 13 | Endocytosis | SD Maintenance |
| AP2M1 | AP-2μ | 13 | Endocytosis | SD Maintenance |
| AP2S1 | AP-2σ | 14 | Endocytosis | SD Maintenance |
| AUXILIN | Aux | 11 | Endocytosis | SD Maintenance |
| HSPA8 | Hsc70-4 | 12 | Endocytosis | SD Maintenance |
| PICALM (AP180) | Lap | 12 | Endocytosis | SD Maintenance |
| RAB5B | Rab5 | 13 | Endocytosis | SD Maintenance |
| RBSN | Rbsn-5 | 11 | Endocytosis | SD Maintenance |
| PIK3C3 (VPS34) | Pi3K59F (Vps34) | 15 | Endocytosis | SD Maintenance |
| GAPVD1 | Gapvd1 | 14 | Endocytosis | SD Maintenance |
| AMN | dAmn | 11 | Endocytosis | SD Maintenance |
| CUBN | Cubn | 12 | Endocytosis | SD Maintenance |
| CUBN | Cubn-2 | 9 | Endocytosis | SD Maintenance |
| RPH3a | Rph | 10 | Endocytosis | SD Maintenance |
| RAB3A | Rab3 | 12 | Endocytosis | SD Maintenance |
| RAB11A | Rab11 | 13 | Recycling | SD Maintenance |
| EXOC1 | Sec3 | 14 | Recycling | SD Maintenance |
| EXOC2 | Sec5 | 14 | Recycling | SD Maintenance |
| EXOC3 | Sec6 | 14 | Recycling | SD Maintenance |
| EXOC4 | Sec8 | 14 | Recycling | SD Maintenance |
| EXOC5 | Sec10 | 14 | Recycling | SD Maintenance |
| EXOC6 | Sec15 | 13 | Recycling | SD Maintenance |
| EXOC7 | Exo70 | 15 | Recycling | SD Maintenance |
| EXOC8 | Exo84 | 14 | Recycling | SD Maintenance |
| TBC1D8B | Tbc1d8b | 13 | Recycling | SD Maintenance |
DIOPT, Drosophila RNAi Screening Center (DRSC) integrative ortholog prediction tool (Hu et al., 2011); SD, slit diaphragm.