Figure 1.

Endothelial-progenitor-cells-specific deletion of Ccm3 results in progressive formation of lesions throughout the brain

Procr^{CreERT2−IRES-tdTomato/+/}Ccm3^f/f mice received a single dose of tamoxifen at P1 and were analyzed at the indicated times.

(A and B) Representative photographs of whole brains (A) and tiling of brain sections (B) stained for PECAM1, showing lesion burden and distribution. Black arrowheads, lesions. Scale bars: 1000 μm.

(C) Quantification of total lesioned area. Data are means ± SE. Each dot represents an animal; p < 0.0001 among groups (ANOVA); ∗p < 0.05, ∗∗p < 0.01 (Tukey’s post hoc tests).

(D) Quantification of lesion numbers. Data are means ± SE. Each dot represents an animal; p < 0.001 among groups (Kruskal-Wallis tests); ∗p < 0.05, ∗∗p < 0.01 (Dunn’s post hoc tests).

(E) Quantification of distribution of lesion area between cerebellum and cerebrum. Data are means ± SE. Each dot represents an animal; p < 0.001 among groups (ANOVA) ∗p < 0.00 1 (Tukey’s post hoc tests).

(F) Quantification of distribution of lesion numbers between cerebellum and cerebrum. Data are means ± SE. Each dot represents an animal; p < 0.001 among groups (Kruskal-Wallis tests) ∗p < 0.05, ∗∗p < 0.01 (Dunn’s post hoc tests).