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. 2022 Feb 9;59:101456. doi: 10.1016/j.molmet.2022.101456

Figure 4.

Figure 4

Differential muscle mitochondrial biogenesis, acylcarnitine accumulation, and fiber enlargement in Cpt2Sk−/−mice is fiber-type-specific. (A1 to A4) Representative Gomori staining of mitochondria (red) in muscle fibers (light blue) of glycolytic (EDL) and oxidative (Soleus) muscles for both genotypes. Far-right column contains magnified images of A2 and A4. Magnification 20X. Scale bar = 100 μm. (B) cytochrome c oxidase protein (COX IV) immunodetection of (B1) control and (B2) Cpt2Sk−/− EDL muscle and (B3) matched myosin heavy chain immunodetection of Cpt2Sk−/− EDL. White arrows indicate the same anatomic structure in B2 and B3. Yellow arrows indicate type IIa myofibers. (C) Digital reconstitution of mass spectroscopy-based lipid scanning (nano-DESI) of linoleoyl-carnitine (C18:2) and matched myosin heavy chain immunodetection of Control (C1,2) and Cpt2Sk−/− (C3,4) TA muscle. (D) Distribution of fiber cross-sectional area (CSA) of control and Cpt2Sk−/− EDL analyzed for Type IIa, IIx, and IIb fibers independently. Data is presented here as the mean of 3 biological replicates per fiber type and genotype.