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Cat MDR1 genotyping. (A) Gene-specific primers and allele-specific probes of the TaqMan AD assay. (B) AD plot revealed discrete clusters for all three genotypes. (C) Fluorescence readouts from the real-time PCR amplification revealed clear separation between fluorescence signals from the VIC-coupled wild-type probe and the FAM-coupled mutant probe. (D) Verification of the TaqMan AD genotyping calls by Sanger sequencing.
