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. 2022 Feb 21;8:808392. doi: 10.3389/fvets.2021.808392

Table 1.

Primer and probe sequences used for cat MDR1 PCR amplification and genotyping.

Forward primer (5' → 3') Allele-specific probes (5' → 3') Reverse primer (5' → 3') Fragment (bp)
ACA AGA GGA AAT GAA ATT GAA TTA GAA AAT GCA VIC-CAA TTT CAC TTA TGG ATT CAT-NFQ MDR1(+) CTG GAT CCT GAA TCT TTT GGA GAC A 93/95
FAM-CAT CAA TTT CAC TTA TGT TCA T-NFQ MDR1(-)
GGT CCA AAG CAA ATA GGA TTG TGT CTC CAC AAA CAT TCA ACC 823