FIGURE 1.

Forced aggregation of ASC in imprinted wells of agarose. (A) Microscopic view of imprinted agarose microwells. (B) Macroscopic view of a thin agarose mould on the hemi permeable membrane in its support. (C) Technical description of the method: forced aggregation in agarose combined with air/liquid interface. Spheroids are forced to aggregate, by centrifugation, in non‐adherent microwells. The agarose is on a polytetrafluoroethylene membrane that, by capillarity, attract the culture medium and create a thin film of medium on spheroids, the establishing air/liquid interface conditions. (D) Manufacturing of ASC spheroids of 3 different ASC cell line from 3 different patients by using the method described, after 48 h of aggregation