FIGURE 4.

Analysis of the secreted proteome from spheroids versus ASC in 2D. (A) ASC in 2D or spheroids were cultured in serum‐free medium (=conditioned media) prior mass spectrometry analysis of its protein composition. The number of proteins identified in each sample is presented. (B) Functional protein class analysis was done by PANTHER (Protein ANalysis THrough Evolutionary Relationships) for proteins secreted only in 2D but not in spheroids. (C) Functional protein class analysis was done by PANTHER (Protein ANalysis THrough Evolutionary Relationships) for proteins secreted only in spheroids but not in 2D