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. 2022 Feb 21;13:837497. doi: 10.3389/fimmu.2022.837497

Figure 2.

Figure 2

Baicalein inhibits LPS-induced phosphorylation of PI3K/NFκB axis in microglia. (A) Representative immunofluorescence photomicrographs showing pho-PI3K p55 (red), pho-NFκB p65 (green), and nuclear labeling with DAPI (blue) on BV2 microglial cells that were untreated (Ctrl) or pretreated with vehicle (Veh) or 10 μM baicalein (Ba) for 24 h followed by 3 h LPS stimulation. Insets showing enlarged images of cells with pho-NFκB and DAPI labeling. Scale bar = 20 μm; 10 μm (insert). (B) Western blots showing the expression of pho-PI3K p55 and pho-NFκB p65 and β-actin (loading control) in BV2 cells that were untreated (Ctrl) or pretreated with Veh or Ba for 24 h followed by 3 h LPS stimulation. (C, D) Relative densitometer analysis of Western blots for pho-PI3K (C) and pho-NFκB (D) normalized to β-actin independently in BV2 cells. N = 6 per group. Data were presented as mean  ± SEM. Each dot represented individual replicate. *P < 0.05, **P < 0.01, ****P < 0.0001, one-way ANOVA with Tukey’s multiple comparisons test.