Figure 5.

Baicalein attenuates CD4⁺ Th17 and Th1 cell responses in the retina and superior cervical lymph nodes after I/R insults. (A) Representative FACS plots showing the gated population of CD4⁺ and IL-17A⁺ T cells and (B) their frequency in the retinas from the Veh or 100 μM baicalein (Ba)-treated mice at 2 weeks I/R injury. (C) Representative FACS plot of CD4⁺/IL-17A⁺ T cells and (D) their frequency in the cervical lymph nodes (LNs) of the Veh or Ba-treated mice at 2 weeks post-I/R injury. (E) Representative FACS plots and (F) the quantification of the frequency of CD4⁺/IFNγ⁺ T cells in the cervical LNs of the Veh or Ba-treated mice at 2 weeks post-I/R injury. N = 5 per group. Data were presented as mean  ± SEM. Each dot represented an individual replicate. *P < 0.05, **P < 0.01, Student’s t-test.