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. 2022 Feb 21;10:803061. doi: 10.3389/fcell.2022.803061

FIGURE 6.

FIGURE 6

Transcriptome profiling of NELL2 in human fetal spinal cord cells. (A) UMAP plot of 43,485 cells colored by gene module score of clusters of CNS progenitor, CNS neurons, PNS progenitors, and PNS neurons adopted from human fetal neural cells adopted from https://shiny.crick.ac.uk/scviewer/neuraltube/ (Rayon et al., 2021). (B) Feature plots of markers of NELL2, neural progenitors (SOX2), neurons (NEUN), astrocytes (GFAP), and oligodendrocyte progenitors (PDGFRA). (C) The distribution of NELL2 and DCX expression across the fetal spinal cord cells at different stages of development. (D) Two-dimensional tSNE of 10,361 cells from 3-month-old hSCOs via single-cell RNA sequencing identified by clustering specific transcription factors of dorsal and ventral spinal cord cells. (E) Gene expression profiles of NELL2, neural progenitors (SOX2), neuronal-specified progenitors (DCX), astrocytes (GFAP), and mature oligodendrocyte (MBP). (F) Violin plots showing the NELL2, SOX2, DCX, GFAP, and MBP (as defined by scRNA-seq) across the 13 clusters. The x-axis is log2 expression.