FIGURE 9.

Simulations mimicking the effect of altering the TRPC3 expression on the TRPC3 signaling characteristics based on the data from Purkinje cells. These results are based on the full model of TRPC3 signaling, which along with TRPC3-mediated influx also accounts for cytosolic release of Ca²⁺ from stores and VDCC-mediated influx in the membrane compartment. Thus, Ca²⁺ release and flux contributions are as follows: (1) release from internal stores; (2) TRPC3-mediated influx; and (3) VDCC-mediated influx. These results show the translocation characteristics of the PKCγ and DGKγ molecular pair and dynamics of second messenger DAG and Ca²⁺ during KCl-induced purinergic receptor activation and stimulation of the TRPC3 signaling cascade in PCs. These results show that, in response to a brief 1-min pulse, DAG is generated at the membrane, thus activating TRPC3 channel, which in turn allows the calcium flux into intracellular space and stimulates the translocation of PKCγ and DGKγ from cytosol to membrane. (A) M/C ratio of PKCγ; (B) M/C ratio of DGKγ; (C) Ca²⁺ temporal dynamics in membrane compartment; (D) Ca²⁺ temporal dynamics in cytosolic compartment; (E) DAG temporal dynamics.