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. 2022 Feb 21;13:839489. doi: 10.3389/fimmu.2022.839489

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Copyright © 2022 Yu, He, Cao, Aleem, Yan, Xu, Song and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Double digestion analysis of the recombinant plasmid TgP2-pVAX1. Line M, DL5000 marker; Line 1, double digestion with EcoRI and XhoI. (B) Immunofluorescence analysis of the expression of TgP2-pVAX1 in HEK 293-T cells. Bar represents 25 μm. (C) Western blotting of cell lysates probed with rat polyclonal antibodies against Toxoplasma gondii STAg. Cell lysates of HEK 293-T cell transfected with TgP2-pVAX1 (Line 1) and pVAX1 (Line 2) plasmids. Line M, MW marker proteins.