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. 2022 Feb 21;13:839489. doi: 10.3389/fimmu.2022.839489

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Copyright © 2022 Yu, He, Cao, Aleem, Yan, Xu, Song and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine secretions of IFN-γ (A), IL-4 (B), IL-10 (C), and IL-17 (D) in animals’ sera at weeks 0, 2, and 4. Commercially available ELISA kits were used to determine the level of cytokines in the sera. Each serum was conducted once, and significance was evaluated by one-way ANOVA followed by Dunnett’s test. Values among the TgP2-pVAX1, TgP2-pVAX1/PLGA, and TgP2-pVAX1/CS groups were pairwise compared by ANOVA following Bonferroni’s correction. Values are shown as mean ± SD (n = 5). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with blank or control group.