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. 2022 Feb 21;13:839489. doi: 10.3389/fimmu.2022.839489

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Copyright © 2022 Yu, He, Cao, Aleem, Yan, Xu, Song and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of dendritic cell (DC) maturation. Five animals in each group were sacrificed, and spleen lymphocytes from each animal were investigated. The bar graph shows the ratio of CD83 (A) and CD86 molecules (B) on splenic DCs, while the dot plots (C) show the percentages of CD11c⁺CD83⁺ and CD11c⁺CD86⁺ cells. Values are shown as mean ± SD (n = 5), and significance was evaluated by one-way ANOVA followed by Dunnett’s test. Values among the TgP2-pVAX1, TgP2-pVAX1/PLGA, and TgP2-pVAX1/CS groups were pairwise compared by ANOVA following Bonferroni’s correction. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with blank or control group.