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. 2022 Jan 9;13(4):1145–1159. doi: 10.7150/jca.63635

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Expression and role of C4orf19 in colorectal adenocarcinoma (COAD). (A) Representative images of positive staining of C4orf19 (marked by red arrows) in normal tissues (upper) and negative staining of C4orf19 in cancer tissues (lower). (B) The expression of C4orf19 in colorectal cancer cells tested by western blot. (C) The expression of C4orf19 protein after transfecting with siRNA for 48h; (D) Effects of C4orf19 siRNA on cell proliferation, *P < 0.05, * P < 0.001. (E, F) Effects of C4orf19 siRNA on cell migration (E) and invasion (F). (G) Western blot analysis was performed for CA2 and CA4 expression in caco2 cells after transfection with C4orf19 siRNA or negative control.