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. 2022 Feb 7;13(4):1313–1323. doi: 10.7150/jca.65444

Figure 4.

Figure 4

PHLPP2 may play a major role in regulating the biological characteristics of CRC cells through the Nrf2-ARE pathway. (A) Transfection efficiency observation with a fluorescence microscope. (B) PHLPP2 protein expression was detected by Western blot. (C) Genes that may be affected by changes in the expression of PHLPP2. (D) Differential expression analysis after interference with PHLPP2. (E) PCR validation of key antioxidant genes. (F) Heatmap analysis of genes containing antioxidant promoters. (G) Enrichment analysis of Nrf2-related genes after PHLPP2 overexpression.