Figure 4.

AHR deficiency alters the transcriptome of intestinal eosinophils. (A) RNA sequencing of small intestinal eosinophils from WT and Ahr^–/– mice was performed in n = 3 males and n = 3 females per genotype. (B) Principal component analysis. (C) Volcano plot of differentially expressed genes between WT and Ahr^–/– eosinophils. (D) GSEA of WT versus Ahr^–/– eosinophils. (E and F) Examples of functionally defined gene subsets from the leading edge of the analysis in D. (G and H) Examples of functionally defined gene subsets identified by DAVID (Fig. S3, E and F) based on differentially expressed genes between WT and Ahr^–/– eosinophils (|FC| > 2, adjusted P value <0.05). (E–H) Shown is the log₂(FC) to the geometric mean of TPM + 1. (I) Genes with differential expression between WT and Ahr^–/– were manually selected and analyzed by qPCR in BMDEo. Correlation of RNA sequencing results with qPCR results for WT versus Ahr^–/– BMDEo. Shown is log₂(FC). qPCR data are from one experiment conducted in technical triplicates.