Figure 7.

AHR-deficient eosinophils show increased degranulation in vivo. (A) Mean fluorescence intensity (MFI) was determined by flow cytometry. Data are pooled from two independent experiments with n = 4 mice per genotype each. **, P < 0.01, unpaired t test. (B) EM of the duodenum of WT and Ahr^–/– mice. Representative images show examples of eosinophils and granules. White scale bar: 2 μm, black scale bar: 100 nm. (C) Granules were counted in 10–15 cells/mouse on EM images, and data are pooled from two mice per genotype. The number of granules was normalized to the area of cytoplasm (cell area − nucleus area). Unpaired t test, P = 0.0582. (D) Gene expression in BMDEo at different time points in culture was determined by qPCR and normalized to Hprt. Shown is the mean ± SEM of four independent experiments. (E) RNA sequencing data of small intestinal eosinophils from n = 6 mice per genotype. (F–H) MFI and surface expression were determined by flow cytometry. Data are pooled from three independent experiments with n = 3–6 mice per genotype (F and G) or from one experiment (H). **, P < 0.01; ***, P < 0.001, unpaired t test. TPM, transcripts per million.