Fig. 5.

OPA1 cleavage and apoptotic death of T-ALL cells induced by everolimus. Jurkat and T-ALL PDX cells were treated with the indicated concentrations of everolimus for 24 h. (A) Detection of OPA1 cleavage. Shown are representative immunoblots of OPA1 isoforms in Jurkat cells and PDX cells; OPA1 ratios were calculated by dividing the intensity of the cleaved OPA1 bands by the intensity of all OPA1 bands and scaled against the value obtained for the untreated sample. (B) Changes in mitochondrial membrane potential. Control and everolimus-treated Jurkat and PDX19 cells were incubated with 5 nM TMRM for 15 min and analyzed by flow cytometry. Resulting histograms show a dose-dependent increase in the number of depolarized cells (gate B). (C) Apoptosis measurements. Scatter plots show flow cytometry analyses of Jurkat and PDX19 cells stained with Annexin V/PI.