Figure 3.
Speed and wave diversity increases as anesthesia fades out
(A) Top: representative distribution of time lags (i.e., rows of the TLM) in the space of the first two principal components (PC1, PC2). All transitions from experiments with WEI ranking one to eight are shown. Magenta contours represent 66% isodensity levels of the distribution. Bottom: 65% isodensity contours for the activation waves from experiments and anesthesia levels pooled in three equally-sized groups according to the WEI ranking.
(B) Activation waves (dots) for all experiments projected on the first 2 PCs versus WEI. Red shading: smoothed 65% isodensity surface. The colored bars on the right show the WEI ranges that define the three groups used in panel (A). The number written on each bar shows the number of PCs needed to obtain a value of Pearson correlation between the TLM reconstructed with this very number of PCs only and the original, full TLM.
(C) Average and single-channel MUA for three experiments and anesthesia levels representative of the WEI groups in (A) and (B). Right: resulting average spatiotemporal propagating patterns of activation waves clustered in different “modes of propagation” (see also supplemental movies S1 and S2).
(D) Number of propagation modes per experiment and anesthesia level. Red line: linear regression (P < 0.05).
(E) Direction and velocity of the modes of propagation from the experiment’s eight lowest and highest values of WEI. Modes are colored according to the results of k-means clustering. Clusters with less than three patterns are not shown (number of excluded modes = 1, number of excluded patterns = 2). Thick arrows: average directions and velocities of each mode.
(F) Mean propagation velocity for each experiment and anesthesia level. Red line: linear regression (P < 10˗4).