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. 2022 Feb 21;13:828269. doi: 10.3389/fphar.2022.828269

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Copyright © 2022 Sachetto, Miyamoto, Tashima, de Souza and Santoro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) LC-MSE chromatogram of rutin (10 ng, RT 6.84). (B) CID-MS/MS spectrum of rutin: main fragment ions observed at m/z 303.06+ and 465.12+. Remaining precursor ion at 611.18+ due to the DIA analysis. Proposed fragmentation routes of (C) rutin and (D) RS by ESI-CID-MS/MS. (E) LC-MSE chromatograms of RS (500 ng) with multiple succinylations (1–6), and (F) representative CID-MS/MS spectrum of RS with 2 and 3 succinylations, at RT 7.924. Remaining precursor ions (at m/z 711.17+ and m/z 811.19+) due to the DIA analysis.