Figure 2.

Dgat2-1473 provides strong silencing for at least 12 weeks after a single subcutaneous injection in the livers of male C57BL/6 mice

(A) Representative cartoon of chemically modified, GalNAc-conjugated siRNAs that were used for in vivo studies. (B) Dose response relationships and longevity of silencing elicited by Dgat2-1473 that was subcutaneously injected into wild-type C57BL/6 mice (n = 3) on chow diet. The mice were treated with the indicated doses (10, 3, 1 mg/kg) of Dgat2-1473 or NTC compound (10 mg/kg) and sacrificed at the indicated time points (4, 8, 12 weeks) after injections. The mRNA levels of Dgat2 were normalized against the NTC group and 18S was used as the housekeeping gene for the calculations. (C) DNL-related gene expression changes upon Dgat2 silencing in wild-type C57BL6 mice. ∗p < 0.05, ∗∗p < 0.005.