Figure 2.

circ-FoxO3 inhibits OGD/R-induced endothelial barrier permeability

The siRNA targeting of circ-FoxO3 or LV-circ-FoxO3 was transfected into the BMECs (bEnd.3 and HBMEC). (A) The relative level of circ-FoxO3 or FoxO3 mRNA was measured by qRT-PCR in BMECs. n = 3. (B) The level of circ-FoxO3 and FoxO3 mRNA was compared in BMECs with circ-FoxO3 overexpression. n = 4. (C) The infiltration of FITC-dextran (10 kDa) across the monolayer of BMECs was measured to determine the role of circ-FoxO3 on the properties of the endothelial cell barrier. n = 6. (D) Representative photomicrographs of tube formation in BMECs with circ-FoxO3 knockdown or overexpression. n = 6. Scale bar, 200 μm. (E) Quantification of the number of tubes in (D). The p values indicate one-way ANOVA with Dunnett's multiple comparisons test. OGD/R, oxygen-glucose deprivation/reoxygenation; O3/R3, oxygen-glucose deprivation for 3 h and reoxygenation for 3 h (O6/R6 and O9/R9 indicate the same for 6 and 9 h, respectively). Data are provided as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.