Figure 3.

circ-FoxO3 attenuates BBB damage in MCAO/R mice

(A) The flowchart of the experimental procedures in mice. (B) The schematic diagram of LV injection (top panel) and the localization of LV in brain tissues of mice (lower panel). White arrowhead indicates that the LV-EGFP-shcirc-FoxO3 or LV-EGFP-circ-FoxO3 localized in vessels. Scale bar, 50 μm. (C) The expression of circ-FoxO3 in the peri-infarct area of mice, n = 4. (D) Representative images of Evans blue extravasation into entire brains of mice with circ-FoxO3 knockdown or overexpression. The white dotted lines in the top panels highlight the injection site of LV. n = 5. Scale bar, 2 mm. (E) Vascular permeability was detected by measurement of Evans blue extravasation in brain tissue using spectrophotometry at 610 nm. n = 5. (F) The cell-cell junctions were imaged by TEM analysis in brain tissue after circ-FoxO3 knockdown or overexpression. n = 5. Scale bar, 250 nm. L, lumen; MCAO/R, middle cerebral artery occlusion for 2 h and followed by reperfusion for 2 h. p values indicate one-way ANOVA with Dunnett's multiple comparisons test. All data are presented as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.