Figure 5.

circ-FoxO3 promotes autophagy in OGD/R-treated BMECs

Autophagy was detected in the monolayer of endothelial cells (bEnd3 and HBMEC) with circ-FoxO3 knockdown or overexpression after OGD/R. (A) Representative images show LAMP1 and LC3B staining. (B) The quantitative integrated optical density of LAMP1 and LC3B in (A). n = 6. Scale bar, 10 μm. (C) Immunoblotting analysis of LC3B II/I and SQSTM1 levels. OGD/R, oxygen-glucose deprivation for 3 h followed by reoxygenation for 3 h. (D) Quantitative results of the bands in (C). n = 4. p values indicate one-way ANOVA with Dunnett's multiple comparisons test. Data are presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.