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. 2022 Feb 20;12(4):e4335. doi: 10.21769/BioProtoc.4335

Table 2. Sequences of the Atto565-labeled DNA probes used in the northern blot experiment.

One probe contains degenerate bases, thus recognizing both tRNAGlyCCC and tRNAGlyGCC. The probes are labeled at their 5’ ends with Atto565.

Probe Sequence
tRNAGlyTCC 5’-CCCGGGTCAACTGCTTGGAAGGCAGCTAT-3’
tRNAGlyCCC/GCC 5’-GYCTCCCGCGTGGSAGGCGAG-3’