Figure 9.
Preparation of bifunctional nanoparticles and evaluation of the peptide brain targeting function. (A) Schematic illustration of the preparation of IS@NP/KHs. Both Aβ42 oligomer affinity peptide KH and brain targeting peptide IS were identified from phage display. Subsequently, IS peptide and KH peptide were assembled into bifunctional NPs (IS@NP/KHs). (B) Phage immunofluorescence staining of the brain sections of APP/PS1 mice. TBST, phage library, and phage clone encoding IS peptide were injected into APP/PS1 mice through the tail vein, respectively, and anti-M13 antibody immunofluorescence staining was performed on the brain sections (phage, green; nuclei, blue). (C) Ex vivo imaging of FITC-IS in main organs. APP/PS1 mice were injected with FITC-labeled IS via a vein tail route. Optical images showing the brain, lung, liver, spleen, and kidney of FITC-IS administered mice after 2 h. Reprinted with permission from 59. Copyright 2021 American Chemical Society.