Figure 5.

Knocking down FoxP3 promotes Parkin-mediated mitophagy in cardiomyocytes. (A) NRVMs were treated, and the levels of Parkin and ATF4 were determined as shown in Figure 3A (n = 3). (B) H9c2 cells and NRVMs were treated with AngII (1 μM) with or without TP (10 μg/L) for 4 h. Nuclear lysates were extracted for immunoblotting (IB) using antibodies against ATF4 and histone H3 (internal control). (C) H9c2 cells grown in 12-well plates were transfected with control siRNA and FoxP3 siRNA and then treated as shown in B for 15 min. FoxP3 or Parkin mRNA expression was determined as shown in Figure 1D. (D-E) H9c2 cells grown on glass slides were transfected and treated as shown in C. Mitochondrial (red), LC3 (green) and Parkin (light blue) staining and colocalization (white) were performed as shown in Figure 3I. ^*P < 0.05, ^**P < 0.01.