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. 2022 Feb 14;12(5):2205–2231. doi: 10.7150/thno.70756

Figure 10.

Figure 10

Hbα knockdown abolishes PBM treatment's protection on primary culturing neurons. (A) Cell viability was measured using dual-fluorescent FD/PI assay. (a) Representative confocal microscopy images of FD/PI staining of primary cortical neurons and hippocampal neurons. Cellular viability was expressed as the ratio of FD/PI (n=8). Scale bar = 20 µm. (B) MTT assay was performed to test cellular metabolic activity as an indicator of cell viability. Data was quantified as percentage changes versus the cont. group. (C) Levels of cleaved-caspase 3 were measured using immunofluorescence staining. Scale bar = 20 µm. n=6. (D) The caspase-3 and (E) caspase 9 activity was measured using the corresponding activity assay kit and expressed as a percentage change versus the control group (n=8). (H) Western blotting and quantitative analyses of Bax and Bcl-1. Data were presented as a percentage change versus the control group (n=5). *P < 0.05 versus control group, # P < 0.05 versus PBM group. ns indicates no significant difference (P > 0.05).