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. 2022 Jan 31;12(5):1952–1970. doi: 10.7150/thno.65539

Figure 3.

Figure 3

Incubation with mC5C2 and GST-cCPEm reveals differences in the reduction of the absolute TEER in eGFP-hCldn5-MDCK II cells. (A-B) Schematic diagram showing mC5C2 binding of the extracellular loop 1 (ECL1) and cCPEm binding ECL2 of claudin-5. The homology model of claudin-5 was created in Swiss-Model using human claudin-9 (PDB ID 6OV2) as template. The cCPEm structure was extracted from the same PDB entry (6OV2). mC5C2 was placed in proximity to the model of claudin-5 whereas cCPEm was docked to claudin-5 for schematic purposes only. The molecular structures were generated using Maestro (Schrödinger Release 2020-4, New York, 2020). (C-D) Incubation of eGFP-hCldn5-MDCK II with mC5C2 and GST-cCPEm causes concentration- and incubation-time-dependent reductions in TEER. N=6 of each condition. Two-way ANOVA with Sidak's multiple comparison test (*p<0.05, and ***p<0.001). Asterisks refer to the difference in TEER value at the measured time points, compared to the TEER value of untreated control.