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. 2022 Jan 7;30(3):1188–1200. doi: 10.1016/j.ymthe.2022.01.007

Figure 1.

Figure 1

Phenotypic characteristics of CD8+ T cells treated with D2HG

(A) Flowchart showing the strategy for experimental D2HG treatment on CD8+ T lymphocytes.

(B) Representative FACS plots showing CD8 and CD45RO with gating selection of activated T lymphocytes.

(C) Representative FACS plots showing CFSE with gating selection of proliferating cells after different concentration of D2HG treatment for 3 days from 3 biological replicates for each group.

(D) Histogram showing the cell proliferating differences after gradient final concentration of D2HG.

(E) Representative FACS plots showing 2-NBDG with gating selection of glucose uptake after different concentration of D2HG treatment from 3 biological replicates for each group.

(F) Histogram showing the glucose uptake differences after gradient final concentration of D2HG.

(G) Representative FACS plots showing CD45RO versus CD62L for the memory subset after D2HG treatment of 500 μM from 3 biological replicates for each group. Differences of memory cell subsets are labeled in the histogram.

(H) Representative FACS plots showing CD8 versus the intracellular cytokines IFN-γ, TNF-α, and lytic granule protease granzyme B after 500 μM D2HG treatment from 3 biological replicates for each group. Differences are labeled in the histogram.

(I) Representative FACS plots showing PD-1 and Tim-3 for CD8 T cells after 500 μM D2HG treatment from 3 biological replicates for each group. Differences are labeled in the histogram. The data and statistical results are in Table S1.