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. 2022 Feb 21;13:752189. doi: 10.3389/fimmu.2022.752189

Figure 4.

Figure 4

IL-10 was a major effector of RNASE2. (A–G) The expression of multiple cytokines (IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-21 and TNF-α) in cultured supernatant was determined by using luminex liquid phase chip, among which only IL-10 level was significantly decreased in RNASE2 silencing group (n=11). The lower limit of detection (LLOD) for each cytokine was 2.96 pg/ml, 5.61 pg/ml, 0.72 pg/ml, 0.74 pg/ml, 1.26 pg/ml, 2.69 pg/ml and 0.98 pg/ml respectively, and levels lower than LLOD were regarded as having the minimum detection value. (H) Independent sample validation by ELISA confirmed the reduction of IL-10 level in cultured supernatants after RNASE2 silencing (n=8) (LLOD 0.05pg/ml). (I) In vivo study showed the level of plasma IL-10 was increased in SLE patients (n=16) compared with healthy controls (n=14). (J) Plasma IL-10 levels in SLE patients were closely linked to peripheral RNASE2 mRNA expression (n=10). Data are presented as mean ± SEM, *p < 0.05, **p < 0.01.