Figure 1.

Pertussis toxin (PT) increases stroke severity in intracerebral haemorrhage (ICH) mice. ICH was induced by injection of 0.0375 U collagenase. After surgeries, mice were treated with PT immediately by intraperitoneal injection at a dose of 250 ng or 500 ng or vehicle. Mice were subjected to neurological assessment and MRI scanning until day 7 after ICH. (A, B) Cumulative data illustrate the neurological assessments of ICH mice and sham mice receiving PT or vehicle from day 1 to day 7 after surgery, including modified Neurological Severity Score (mNSS) (A) and rota-rod test (B). n=8 mice per group from three independent experiments. Data were expressed as mean±SEM; *p<0.05; **p<0.01. (C) Representative MR images show lesion area and haematoma area in ICH mice receiving PT or vehicle. Red lines delineate lesion area, yellow shadows represent haematoma area and perihaematomal oedema volume was calculated by subtracting the haematoma volume from lesion volume. (D) Cumulative data show the lesion volume and perihaematomal oedema volume of ICH mice receiving PT or vehicle. n=4 mice per group from two independent experiments. Data were expressed as mean±SEM; *p<0.05.