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. 2021 Aug 2;7(1):29–37. doi: 10.1136/svn-2021-000987

Figure 3.

Figure 3

Pertussis toxin (PT) promotes brain-infiltrating leucocytes after intracerebral haemorrhage (ICH). ICH was induced by 0.0375 U collagenase injection. At day 1, day 3 or day 7 after ICH, single cell suspensions were isolated from brain tissues of ICH mice treated with PT or vehicle. (A) Gating strategy of brain-infiltrating immune cells including neutrophils (CD45highCD11b+Ly6G+), CD4+ T cells (CD45highCD3+CD4+), CD8+ T cells (CD45highCD3+CD8+), B cells (CD45highCD3−CD19+), and NK cells (CD45highCD3−NK1.1+). (B) Quantification of lymphocytes, monocytes and neutrophils in the brain of sham and ICH mice receiving PT or vehicle at indicated time points. Day 1 after ICH, n=3 mice per group from two independent experiments; day 3 and day 7 after ICH, n=5 mice per group from three independent experiments. The data were calculated as mean±SEM; *p<0.05.